Nikon TIRF microscope
On a Nikon Ti-E inverted microscope
(MP110, Molson Pavillion)
Light Sources Objectives Fluorescence
Filter cubes
Features
Halogen lamp
(Brightfield)
Super Plan Fluor
ELWD 20 x
FITC/Cy2/GFP Motorized
x,y stage
X-Cite metal
halide light source
(Fluorescence)
Plan Apo TIRF
60x H, N.A. 1.49
TRITC/Cy3/RFP Nikon TIRF module
operated by NiS Elements
software
Diode lasers:
405 nm, 100 mW
488 nm, 50 mW
561 nm, 50 mW
640 nm, 40 mW
Plan Apo TIRF
100x H, N.A. 1.49
  iXON+ DU897 backthinned
EMCCD camera,
512 × 512 pixel,
16 µm array
    Heated life cell
stage insert, CO2 superfusion
possible, software controlled
      Camera triggering from
the AOTF for high
speed (30 fps)
      Allows limited
dSTORM and PALM

Reservation: Online reservation scheduler on this site
Data transfer: DVD; USB