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Ca++-imaging/Life-Cell
setup on Nikon TE 2000-U inverted microscope (Rm 889 Penfield) |
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| Light Sources | Objectives |
Fluorescence Filter cubes |
Features |
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Halogen lamp (Brightfield) |
Plan
Fluor 10×/0.30 Ph I DLL |
DAPI |
Cell
Fully motorized stage (x,y,z) |
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Xenon
100 W (Fluorescence) |
Plan
Fluor 20×/0.45 DIC L |
FITC/Cy2/GFP |
Ratiometric Ca++ imaging (FURA-2) |
| S Fluor 40×/1.30 Oil | TRITC/Cy3/RFP |
Sutter Lambda 10-2 filter changer and shuttering system |
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Plan
Fluor 60×/0.50 – 1.25 Oil Iris DIC H |
Texas Red |
Life
cell time lapse imaging |
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Plan
Fluor 100×/1.30 Oil Ph3 DLL |
Cy5 |
Photometrics Coolsnap HQ cooled CCD |
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| Operated by Metamorph and Metafluor software | |||
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A
separate computer running the same software is available for offline data analysis (Rm 880) |
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Reservation: Signup Sheet at Microscope |
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