Ca++-imaging/Life-Cell setup
on Nikon TE 2000-U inverted microscope
(Rm 889 Penfield)
Light Sources Objectives Fluorescence
Filter cubes
Features
Halogen lamp
(Brightfield)
Plan Fluor 10/0.30
Ph I DLL
DAPI Cell Fully motorized
stage (x,y,z)
Xenon 100 W
(Fluorescence)
Plan Fluor 20/0.45
DIC L
FITC/Cy2/GFP Ratiometric Ca++
imaging (FURA-2)
  S Fluor 40/1.30 Oil TRITC/Cy3/RFP Sutter Lambda 10-2
filter changer and
shuttering system
  Plan Fluor 60/0.50
1.25 Oil Iris DIC H
Texas Red Life cell time lapse
imaging
  Plan Fluor 100/1.30
Oil Ph3 DLL
Cy5 Photometrics Coolsnap
HQ cooled CCD
      Operated by Metamorph and Metafluor software
      A separate computer
running the same software
is available for offline data
analysis (Rm 880)

Reservation: Signup Sheet at Microscope
Data transfer: DVD; USB